Epitranscriptomic analysis of m⁶A methylome in rats after lumbosacral nerve root avulsion

Background: N⁶-methyladenosine (m⁶A) is the most prevalent modification in mRNAs but its role in lumbosacral nerve root avulsion (LSRA) remains elusive. Materials & methods:Mettl3 expression and global m⁶A level were detected by qPCR, western blot and immunostaining. Altered m⁶A-tagged transcript profiles were revealed by methylated RNA immunoprecipitation and RNA sequencing. Results:Mettl3 and global m⁶A level were upregulated in spinal cord tissues of LSRA rats. In all, 1087 m⁶A peaks were differentially modified by m⁶A, of which 654 were upregulated and 433 downregulated. Biological functions of these transcripts and the hypermethylated or hypomethylated transcripts were also identified. Conclusion: Our findings revealed a profound function of m⁶A modification in LSRA, which provides new insights into its pathogenesis.