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A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells
Ya Zhang1, Huishan Li1, Hailing Mai1
1Guangdong Provincial Key Laboratory of, Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen 529020, China. wyuchemlihg@126.com.
Abstract:
In this study, we designed and tested fluorescent probe zy-2 for specific and responsive imaging of pyruvate kinase M2 (PKM2), which can be excited by 419 nm light. A 17-fold enhancement in the responsive emission upon zy-2's binding to PKM2 was observed, imaging of which was successfully recorded in a time- and concentration-dependent manner in PKM2-positive cells. Thus, we obtained a responsive fluorescent probe for the specific and sensitive detection of PKM2, which is innovative in design and applicable to the detection of cancer cells.
