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Updated: Sep 23, 2025

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
Published on: September 5, 2013
Possibilities of transmission electron microscopy in assessing the quality of bacterial cells and microbial
V N Gerasimov1, N N Marinina1, Galina Georgievna Kharseeva2
1Federal Budgetary Institution of Science State Research Center for Applied Microbiology and Biotechnology.
Abstract:
Determination of changes in the morphostructure of microorganisms under the influence of antibiotics, disinfectants, biocins, physical and chemical agents is carried out using the method of visualization of ultrathin sections in a transmission electron microscope. The aim is to develop methodological techniques for express preparation and visualization of the ultrastructure of bacteria in a transmission electron microscope to detect structural damage to cells and assess the quality of the microbial population. A technique of accelerated sample preparation for visualization of the fine structure of bacteria and diagnosis of structural damage to cells in a transmission electron microscope has been developed. The use of this method will make it possible to more effectively investigate the mechanisms of inactivation of microorganisms at different stages of contact with antibiotics, disinfectants, biocines, including various stressful, damaging and lethal physical and chemical effects.
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