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Updated: Sep 23, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.3K

miR-29a-3p mitigates the development of osteosarcoma through modulating IGF1 mediated PI3k/Akt/FOXO3 pathway by

Song Qi1, Li Xu1, Yongyuan Han2

  • 1Department of Trauma Surgery, Wuhan No 1 Hospital, Wuhan, Hubei, China.

Cell Cycle (Georgetown, Tex.)
|May 16, 2022
PubMed

Insights

MicroRNA-29a-3p (miR-29a-3p) is downregulated in osteosarcoma (OS). Restoring miR-29a-3p inhibits OS cell growth and metastasis by promoting autophagy and suppressing the IGF1 signaling pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a highly malignant bone tumor common in children and adolescents.
  • Understanding the molecular mechanisms regulating OS development is crucial for targeted therapies.

Purpose of the Study:

  • To investigate the expression and functional role of microRNA-29a-3p (miR-29a-3p) in osteosarcoma (OS).
  • To elucidate the relationship between miR-29a-3p and Insulin-like Growth Factor 1 (IGF1) in OS progression.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) for gene expression analysis.
  • Cell proliferation, apoptosis, migration, and invasion assays (MTT, colony formation, Transwell).
  • Dual-luciferase reporter assay and Western blot to confirm molecular interactions and pathway activation.

Main Results:

  • miR-29a-3p was significantly downregulated in OS tissues and cell lines.
  • Overexpression of miR-29a-3p suppressed OS cell proliferation, migration, invasion, and colony formation, while promoting apoptosis and autophagy.
  • IGF1 was identified as a direct target of miR-29a-3p, and IGF1 promoted OS progression via the IGF-1R/PI3k/Akt/FOXO3 pathway.

Conclusions:

  • miR-29a-3p acts as a tumor suppressor in OS by inhibiting cell proliferation, migration, and invasion.
  • miR-29a-3p represses OS development by inducing autophagy and suppressing the IGF1-mediated PI3k/Akt/FOXO3 pathway.
  • Targeting miR-29a-3p or the IGF1 pathway may offer therapeutic strategies for osteosarcoma.
Keywords:
IGF1miR-29a-3posteosarcomaprogressionsignaling pathway

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