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Updated: Aug 13, 2026

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
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Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

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Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery

Gaochen Lu1,2, Weihong Wang1,2, Pan Li1,2

  • 1Medical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China.

Microbial Biotechnology
|May 16, 2022
PubMed

Insights

Washed microbiota transplantation (WMT) significantly reduces fever incidence in ulcerative colitis patients compared to manual faecal microbiota transplantation (FMT). This study details WMT

Area of Science:

  • Microbiology
  • Gastroenterology
  • Transplantation Medicine

Background:

  • Faecal microbiota transplantation (FMT) practices vary globally in safety, methodology, and delivery.
  • Washed microbiota transplantation (WMT) is an improved FMT method using automated filtration and washing.
  • The China Microbiota Transplantation System (CMTS) provides a centralized, non-profit FMT service.

Purpose of the Study:

  • To document the evolution of FMT preparation from manual to WMT methods (2012-2021) at CMTS.
  • To analyze donor screening success rates, adverse event incidence, and delivery strategies.
  • To establish a standardized WMT unit based on bacterial count rather than fecal weight.

Main Methods:

  • Retrospective analysis of prospectively recorded data from the CMTS.
  • Evaluation of donor screening protocols and outcomes.
  • Comparison of fever incidence between manual FMT and WMT in ulcerative colitis patients.

Main Results:

  • Donor screening success rate was 3.1% (32/1036).
  • Fever incidence in ulcerative colitis patients decreased from 19.4% (manual FMT) to 2.7% (WMT).
  • A standardized WMT unit was defined as 10 cm³ microbiota precipitation (1.0 × 10¹³ bacteria).

Conclusions:

  • WMT demonstrates improved safety and efficacy, particularly for ulcerative colitis treatment.
  • Standardized WMT units based on bacterial load enhance preparation quality control.
  • Colonic transendoscopic enteral tube delivery is a promising method for WMT administration.

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