Multifunctional hydrogels based on oxidized pectin and gelatin for wound healing improvement

Gelatin (G) cross-linked with oxidized pectin (OP) was studied as a potential scaffold material for tissue engineering. The effect of oxidation on the chemical properties of pectin was investigated by determining the carbonyl and carboxyl amounts. The OP treatment led to a significant decrease of all values (Mn, Mw, [η] and Rh) determined by size exclusion chromatography (SEC) coupled on line with multiangle light scattering and viscometer detectors. Cross-linking parameters were elucidated by FTIR and TNBS assay. In general, the degree of crosslinking increased with the oxidation of pectin. It was found that the presence of the crosslinking agents caused a reduction in swelling and in the gelatin release which was determined by the BCA kit assay. From the hemolysis test, the membrane of red blood cells was not disrupted by the contact of films and the rate of release of hemoglobin was lower than 5%. The coagulation properties were evaluated by the dynamic blood clotting test. The G/OP hydrogels manifested a good activity of wound healing in the albino rats' model. Moreover, the films did not produce any unwilling symptoms. So, it was concluded that studied films have the potentiality to be used as wound healing biomaterials.