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Identification of Common Oncogenic Genes and Pathways Both in Osteosarcoma and Ewing's Sarcoma Using Bioinformatics
Jingwei Zhang1, Junchao Huang2, Wenjun Liu1
1Department of Orthopedics, Shanghai Fengxian District Central Hospital/Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China.
Journal of Immunology Research
|May 17, 2022
Summary
Researchers identified common oncogenic genes, FN1, COL1A1, and COL1A2, in osteosarcoma and Ewing's sarcoma. These genes may act as antioncogenes, enhancing cisplatin sensitivity in osteosarcoma cells.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Osteosarcoma and Ewing's sarcoma are aggressive bone cancers with distinct molecular profiles.
- Identifying common oncogenic drivers and pathways is crucial for developing targeted therapies.
Purpose of the Study:
- To explore shared oncogenic genes and pathways in osteosarcoma and Ewing's sarcoma.
- To identify potential therapeutic targets for both bone cancers.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) database for microarray data analysis.
- Identified differentially expressed genes (DEGs) and constructed protein-protein interaction (PPI) networks.
- Validated key gene expression in patient tissues using RT-qPCR and Western blot.
Main Results:
- Identified 201 differentially expressed genes and constructed a PPI network with 121 nodes and 232 edges.
- Highlighted FN1, COL1A1, and COL1A2 as potential hub genes involved in both cancers.
- Observed reduced expression of FN1, COL1A1, and COL1A2 in tumor tissues; their knockdown enhanced U2OS cell proliferation and migration, increasing cisplatin sensitivity.
Conclusions:
- FN1, COL1A1, and COL1A2 are common oncogenic genes in osteosarcoma and Ewing's sarcoma.
- These genes may function as antioncogenes, enhancing cisplatin sensitivity in osteosarcoma.
- Further investigation into these genes could lead to novel therapeutic strategies for bone sarcomas.
