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Updated: Sep 23, 2025

Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
Published on: June 17, 2025
Brevibacillus dissolubilis sp. nov., Isolated from Fresh Water
Wei Li1, Zhao-Xuan Wang2, Fu Ma1
1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Abstract:
A Gram-positive-staining, strictly aerobic, motile, ellipsoidal endospore-forming bacterial strain, designated CHY01T, was isolated from the Chishui river in a section of Maotai Town, Guizhou Province, Southwest China. Strain CHY01T was found to grow optimally at pH 8.0 and 28 °C. The 16S rRNA gene sequence analysis indicated that strain CHY01T belonged to the genus Brevibacillus and clustered with the type strain of Brevibacillus panacihumi, with which it exhibited 16S rRNA gene sequence similarity values of 97.8%. The predominant respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were C14:0, iso-C15:0, anteiso-C15:0, C16:0, C15:1iso-H and/or C13:0 3-OH, and C16:1ω7c and/or C16:1ω6c. Genome sequencing revealed a genome size of 6.1 Mbp and a G + C content of 50.6%. The results of physiological and biochemical tests allowed strain CHY01T to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Pairwise determined whole-genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values suggested that strain CHY01T represents a new species, for which we propose the name Brevibacillus dissolubilis sp. nov. with the type strain CHY01T (= CGMCC 1.15916 T = KCTC 33863 T).
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