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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
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Fluorescence intensity ratio technique and its reliability.

Vishab Kesarwani1, Vineet Kumar Rai1

  • 1Laser and Spectroscopy Laboratory, Department of Physics, Indian Institute of Technology (Indian School of Mines), Dhanbad- 826004, Jharkhand, India.

Methods and Applications in Fluorescence
|May 17, 2022
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Summary
This summary is machine-generated.

This study investigated Er3+/Yb3+ codoped glasses, revealing highly pure green upconversion emission. The developed materials show low thermal quenching, making them suitable for temperature sensing applications.

Keywords:
PRAabsorbancelaser-induced heatinglifetimethermal quenchingtransition probabilityupconversion

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Area of Science:

  • Materials Science
  • Optical Physics
  • Spectroscopy

Background:

  • Upconversion (UC) materials are crucial for applications like bio-imaging and sensing.
  • Erbium (Er3+) and Ytterbium (Yb3+) codoped glasses offer promising optical properties for UC processes.

Purpose of the Study:

  • To synthesize and characterize Er3+/Yb3+ codoped glasses.
  • To investigate their optical absorption and upconversion luminescence properties.
  • To evaluate their potential for temperature sensing applications.

Main Methods:

  • Melt-quenching technique for glass synthesis.
  • X-ray Photoelectron Spectroscopy (XPS) and X-ray Fluorescence (XRF) for elemental and compositional analysis.
  • Optical absorption and upconversion emission spectroscopy.
  • Judd-Ofelt analysis for optical parameter calculation.
  • Temperature-dependent emission spectra and Arrhenius fitting.

Main Results:

  • Confirmed glass composition and elements using XPS and XRF.
  • Calculated Judd-Ofelt parameters, transition probabilities, and radiative lifetimes.
  • Achieved highly pure green emission (94.2%) with non-colour tunability.
  • Established a reliable FIR-based temperature scale using temperature-dependent UC spectra.
  • Demonstrated low thermal quenching of green luminescence.

Conclusions:

  • The synthesized Er3+/Yb3+ codoped glasses exhibit excellent optical properties for green UC emission.
  • The materials show potential for accurate temperature sensing due to low thermal quenching.
  • Judd-Ofelt analysis provides insights into the Er-O bond and radiative properties.