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Updated: Sep 23, 2025
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Mitochondrial dysfunction is involved in the cellular activity inhibition by eleutheroside B in SMMC-7721 and HeLa
Shangfan Sun1,2, Yi Zhang3, Weili Xu4
1School of Basic Medical Sciences, 128790North China University of Science and Technology, China.
Abstract:
Eleutheroside B, also known as syringin, has been shown to have various pharmacological activities such as anti-inflammatory, anti-irradiation and antidepressant, but there are few studies on its anti-cancer activity. Its anti-tumor effect on SMMC-7721 cells has not been revealed. Moreover, whether it induces autophagy is still unclear. Thus, the present study investigated whether Eleutheroside B induces apoptosis, autophagy and cellular motility in SMMC-7721 cells and HeLa cells, and explored the underlying molecular mechanisms. SMMC-7721 cells and HeLa cells treated with Eleutheroside B and cell viability measured by MTT assay and trypan blue dye exclusion assay. Apoptosis checked by flow cytometry combined, fluorescent staining. Apoptotic signal proteins and autophagy proteins were checked by Western blot. This study showed that Eleutheroside B inhibited the cell proliferation and blocked cell cycle, migration and invasion as well. Moreover, Eleutheroside B induced apoptosis in SMMC-7721 cells and HeLa cells. It upregulated Bax expression, while simultaneously decreasing Bcl-2 expression. Further elucidation of the mechanism revealed that Eleutheroside B induced mitochondrial dysfunction, with mitochondrial membrane potential collapse and cytochrome c release, suggesting that Eleutheroside B induced apoptosis by triggering mitochondrial pathway. Most importantly, Eleutheroside B could induce autophagy in SMMC-7721 cells and HeLa cells. Taken together, these results suggested Eleutheroside B is a potential therapeutic candidate for HCC and Human cervical cancer.
Insights
Eleutheroside B, a natural compound, effectively inhibits cancer cell growth, migration, and invasion in liver and cervical cancer models. It also triggers apoptosis and autophagy, indicating its potential as an anti-cancer therapeutic agent.
Area of Science:
- Pharmacology
- Molecular Biology
- Oncology
Background:
- Eleutheroside B (syringin) exhibits anti-inflammatory, anti-irradiation, and antidepressant properties.
- Limited research exists on the anti-cancer activity and autophagy-inducing effects of Eleutheroside B.
- The anti-tumor mechanisms of Eleutheroside B in SMMC-7721 (hepatocellular carcinoma) and HeLa (cervical cancer) cells remain largely unexplored.
Purpose of the Study:
- To investigate the effects of Eleutheroside B on apoptosis, autophagy, and cellular motility in SMMC-7721 and HeLa cells.
- To elucidate the underlying molecular mechanisms of Eleutheroside B's anti-cancer activity.
- To evaluate Eleutheroside B as a potential therapeutic candidate for hepatocellular carcinoma (HCC) and human cervical cancer.
Main Methods:
- Cell viability was assessed using MTT and trypan blue assays.
- Apoptosis was analyzed via flow cytometry and fluorescent staining.
- Western blotting was employed to examine apoptotic and autophagy-related proteins.
- Mitochondrial function, including membrane potential and cytochrome c release, was investigated.
Main Results:
- Eleutheroside B significantly inhibited proliferation, cell cycle progression, migration, and invasion in both cell lines.
- The compound induced apoptosis by upregulating Bax and downregulating Bcl-2 expression, activating the mitochondrial pathway.
- Eleutheroside B triggered mitochondrial dysfunction, evidenced by membrane potential collapse and cytochrome c release.
- Autophagy was successfully induced by Eleutheroside B in SMMC-7721 and HeLa cells.
Conclusions:
- Eleutheroside B demonstrates potent anti-cancer effects against hepatocellular carcinoma and human cervical cancer cells.
- The compound induces cancer cell death through apoptosis via the mitochondrial pathway and also triggers autophagy.
- Eleutheroside B represents a promising therapeutic candidate for treating liver and cervical cancers.
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