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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
548

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Multi-analyte High-Throughput Microplate-SERS Reader with Controllable Liquid Interfacial Arrays.

Baomei Zhou1, Cheng Qu1, Shanshan Du1

  • 1School of Food and Biological Engineering, Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui 230009, China.

Analytical Chemistry
|May 17, 2022
PubMed
Summary
This summary is machine-generated.

A novel microplate-surface-enhanced Raman scattering (SERS) reader enables high-throughput liquid sample analysis. This reader utilizes three-dimensional liquid interfacial arrays (LIAs) for rapid, reproducible, and multiplexed detection in biological and environmental applications.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • High-throughput liquid sample analysis is crucial for biology and environmental monitoring.
  • Existing methods lack efficiency and multiplexing capabilities for complex samples.

Purpose of the Study:

  • To develop a semiautomatic, high-throughput microplate-SERS reader for liquid sample testing.
  • To enable rapid identification and quantification of diverse analytes and concentrations.

Main Methods:

  • Development of a microplate reader integrating three-dimensional liquid interfacial arrays (LIAs).
  • Control of LIA formation (oil-in-water, water-in-oil, 2D plane) via container hydrophilicity.
  • Force analysis of LIA and quantification of organic phase density effects.
  • Semiautomatic detection of 12 samples within 10 minutes with internal standard calibration.

Main Results:

  • Demonstrated formation of LIAs in various states for the first time.
  • Achieved fast, continuous, and reproducible detection with isolated wells preventing cross-talk.
  • Successfully performed high-resolution dual and triple analyte detection in mixtures.
  • Preserved individual analyte signals and Raman features, indicating anticounterfeiting potential.

Conclusions:

  • The microplate-SERS reader effectively combines LIA, microplate, and SERS advantages for analyzing complex liquid samples.
  • This technology offers a powerful tool for high-throughput screening, quantification, and anticounterfeiting applications.