Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 23, 2025

High-throughput Titration of Luciferase-expressing Recombinant Viruses
08:09

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Published on: September 19, 2014

13.0K

Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence.

Kari Kopra1, Nazia Hassan1, Emmiliisa Vuorinen1

  • 1Department of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.

Analytical and Bioanalytical Chemistry
|May 17, 2022
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2026
Same author

Nucleocapsid protein enhances spike- and RBD-specific humoral and cellular immune responses in protein-based SARS-CoV-2 vaccine.

BMC infectious diseases·2026
Same author

Glucose Metabolism and Innate Immune Responses in Influenza Virus Infection: Mechanistic Insights and Clinical Perspectives.

Cells·2026
Same author

Gene expression analysis of diabetic foot ulcers reveals the potential impact of Levofloxacin on wound healing.

Scientific reports·2025
Same author

Influenza A(H5N8) vaccine induces humoral and cell-mediated immunity against highly pathogenic avian influenza clade 2.3.4.4b A(H5N1) viruses in at-risk individuals.

Nature microbiology·2025
Same author

Corrigendum to "Long-term neutralization capacity of vaccine and breakthrough infection induced SARS-CoV-2 specific antibodies against omicron subvariants BA.2, XBB.1.5, and JN.1" [Vaccine 68 (2025) 127894].

Vaccine·2025
This summary is machine-generated.

A new time-resolved luminescence method quantifies virus particles quickly and affordably. This Protein-Probe assay offers a simple way to determine virus counts for diagnostics and vaccine development.

Area of Science:

  • Virology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Viruses pose significant global risks, necessitating accurate quantification for diagnostics and vaccine development.
  • Current virus quantification methods are often complex, costly, and require highly purified samples.
  • Existing assays lack direct virus particle count information.

Purpose of the Study:

  • To develop a simple, cost-effective method for rapid virus particle quantification.
  • To establish a novel assay for determining virus particle counts without extensive purification.
  • To provide a tool for accurate virus enumeration in research and development.

Main Methods:

  • Developed a time-resolved luminescence-based assay using a Eu3+-peptide probe.
  • Implemented a mix-and-measure technique for direct virus particle recognition.
Keywords:
Label-freeProtein-ProbeTime-resolved luminescenceVirus particle quantification

More Related Videos

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

13.0K
Virus Propagation and Cell-Based Colorimetric Quantification
07:26

Virus Propagation and Cell-Based Colorimetric Quantification

Published on: April 7, 2023

1.0K

Related Experiment Videos

Last Updated: Sep 23, 2025

High-throughput Titration of Luciferase-expressing Recombinant Viruses
08:09

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Published on: September 19, 2014

13.0K
Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

13.0K
Virus Propagation and Cell-Based Colorimetric Quantification
07:26

Virus Propagation and Cell-Based Colorimetric Quantification

Published on: April 7, 2023

1.0K
  • Validated the method across diverse enveloped and non-enveloped viral samples.

    • Main Results:

    • The Protein-Probe method provides virus particle counts in minutes.
    • Achieved over tenfold higher detectability for enveloped viruses compared to non-enveloped viruses.
    • Demonstrated a dynamic range from 5E6 to 3E10 viral particles/mL.
    • Successfully quantified multiple types of enveloped and non-enveloped viruses.

    Conclusions:

    • The developed luminescence assay is a robust and efficient tool for virus particle quantification.
    • This method simplifies virus enumeration, making it accessible for diagnostics and vaccine research.
    • The Protein-Probe assay overcomes limitations of current virus quantification techniques.