Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pleiotropic changes in cycloheximide-resistant insect cell clones.

N Nouri, A M Fallon

    In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
    |March 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Motivations and personality characteristics of candidate sperm and oocyte donors according to parenthood status: a national study from the French CECOS network.

    Human reproduction open·2022
    Same author

    Decline in peripheral blood NKG2D+CD3+CD56+ NKT cells in metastatic colorectal cancer patients.

    Bratislavske lekarske listy·2018
    Same author

    An insertion mutation in HOXC13 underlies pure hair and nail ectodermal dysplasia with lacrimal duct obstruction.

    The British journal of dermatology·2017
    Same author

    Surface osteosarcoma: Clinical features and therapeutic implications.

    Journal of bone oncology·2016
    Same author

    [Significant association between nonsyndromic cleft lip with or without cleft palate and IRF6rs2235371 polymorphism in Iranian familiar population].

    Molekuliarnaia biologiia·2015
    Same author

    Wolbachia from the planthopper Laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells.

    In vitro cellular & developmental biology. Animal·2012
    Same journal

    Culturing of primary hepatocytes as entrapped aggregates in a packed bed bioreactor: a potential bioartificial liver.

    In vitro cellular & developmental biology : journal of the Tissue Culture Association·1993
    Same journal

    Fetal bovine serum and other sera used in tissue culture increase epithelial permeability.

    In vitro cellular & developmental biology : journal of the Tissue Culture Association·1993
    Same journal

    Epidermal growth factor and insulin-like growth factor-1 preserve cell viability in the absence of protein synthesis.

    In vitro cellular & developmental biology : journal of the Tissue Culture Association·1993
    Same journal

    Absence of trypsinogen autoactivation and immunolocalization of pancreatic secretory trypsin inhibitor in acinar cells in vitro.

    In vitro cellular & developmental biology : journal of the Tissue Culture Association·1993
    Same journal

    Nickel-induced increases in gap junctional communication in the uterine cell line SK-UT-1.

    In vitro cellular & developmental biology : journal of the Tissue Culture Association·1993
    Same journal

    Extracellular calcium does not contribute to cryopreservation-induced cytotoxicity.

    In vitro cellular & developmental biology : journal of the Tissue Culture Association·1993
    See all related articles

    Cycloheximide-resistant mosquito cell mutants were developed for ribosome studies. These mutants exhibit altered growth and drug sensitivities, offering insights into ribosome function and genetics.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Genetics

    Background:

    • Somatic cell mutants resistant to ribosome-targeting drugs are valuable tools for studying ribosome structure, function, and genetics.
    • Aedes albopictus (mosquito) cells were used to generate drug-resistant mutants.

    Purpose of the Study:

    • To obtain and characterize cycloheximide-resistant mutants from Aedes albopictus cells.
    • To investigate the effects of cycloheximide resistance on cell growth, drug sensitivity, and genetic makeup.

    Main Methods:

    • Induction and selection of cycloheximide-resistant mutants (Cx-705 and Cx-738) from Aedes albopictus cells.
    • Assessment of cycloheximide resistance in cell-free protein synthesis lysates.
    • Evaluation of cell growth at different temperatures and sensitivity to various antibiotics (G-418, puromycin, emetine, cryptopleurine).

    Related Experiment Videos

  • Analysis of nuclear ploidy in mutant cell lines.

    • Main Results:

    • Cycloheximide-resistant mutants (Cx-705, Cx-738) showed approximately 30-fold resistance to cycloheximide.
    • Protein synthesis in cell-free lysates from mutants remained resistant to cycloheximide.
    • Mutant cells exhibited poor growth and lethality at 34.5°C, unlike wild-type cells.
    • Mutants displayed increased sensitivity to G-418 but not to puromycin, emetine, or cryptopleurine.
    • Cx-705 cells were predominantly diploid, while Cx-738 cells showed a high frequency of tetraploid nuclei.

    Conclusions:

    • The identified cycloheximide-resistant mutants possess alterations affecting the ribosome.
    • These mutants present a valuable model for exploring ribosome genetics and function, particularly concerning temperature sensitivity and cross-resistance to other ribosome-targeting antibiotics.
    • The distinct ploidy profiles of the mutants suggest potential roles in their observed phenotypes.