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Related Experiment Videos

Cell cycle-dependent methyl esterification of lamin B.

D Chelsky, J F Olson, D E Koshland

    The Journal of Biological Chemistry
    |March 25, 1987
    PubMed
    Summary

    Protein methyl esterification is widespread, with lamin B demethylation occurring during mitosis. This modification may regulate nuclear envelope dynamics, and its inhibition in brain cells could explain their inability to divide.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • Reversible protein methyl esterification involves approximately 24 proteins in mouse lymphoma cells.
    • Many protein methyl esters are ubiquitous across tissues and species, while others exhibit tissue-specific distribution.

    Purpose of the Study:

    • To investigate the prevalence and characteristics of protein methyl esterification in various mouse tissues and cell lines.
    • To identify specific methylated proteins and elucidate their roles, particularly in relation to cell cycle and tissue-specific functions.

    Main Methods:

    • Analysis of protein methyl esterification across mouse tissues, hamster, and human cell lines.
    • Identification of lamin B as a methyl esterified protein using cellular localization and immunological detection.
    • Quantification of methylation stoichiometry via radioactive incorporation and charge analysis using immunoblotting and isoelectric focusing.

    Main Results:

    • Lamin B, a nuclear envelope protein, is demethylated during mitosis, unlike most other methyl esterified proteins.
    • Demethylation of lamin B correlates with a gain of negative charges, suggesting the conversion of methyl esters to carboxylic acid groups.
    • Lamin B methylation turnover is inhibited in intact mouse brain tissue, though it can occur upon cell lysis.

    Conclusions:

    • Lamin B demethylation during mitosis suggests a role in nuclear envelope disassembly and reassembly.
    • The inhibition of methyl group turnover in brain tissue may be linked to the cell cycle-dependent inability of these cells to divide.

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