Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation

Affinity chromatography is one of the versatile technique to selectively separate target biomolecules from complex biological sources (plasma, saliva, urine, etc.). Conventional chromatography resins possess technical limitations at mini-analytical scale, which was overcome with the use of alternative material known as monoliths. This chapter discusses on the how to modify the fused silica capillary inner surface, prepare polymer monoliths within the capillary confinements, chelation of metal-ions on monoliths and protein separation from diluted human plasma using metal-ion monolith microcolumn.