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Updated: Aug 9, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Development of a microbore high-performance liquid chromatographic system for biological applications
Abstract:
Simple and relatively inexpensive modifications are described for conversion of an existing high-performance liquid chromatographic system to a microbore system capable of isocratic or gradient elution. Use of micro guard columns was shown to be a practical method of protecting the microbore analytical columns when chromatographing samples of physiological origin. The microbore chromatograph was successfully integrated with an autoinjector to permit automation of the system. The modified microbore system was used successfully for the isocratic and gradient separations of standard solutions as well as physiological samples. Evaluation of the system performance was based upon comparisons of column efficiency, selectivity, resolution, and sensitivity.
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