Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peptide Nucleic Acids (PNAs) in Antimicrobial Therapy: A Next Generation Strategy.

International journal of molecular sciences·2026
Same author

Se nanoparticles-coated, PLGA-based spheres for biomedical applications: Cytotoxicity, genotoxicity, oxidative stress, biodistribution, and scintigraphic study.

Journal of biomaterials applications·2026
Same author

Hyaluronic Acid-Palmitate Nanoparticle Delivery of Carbonic Anhydrase Inhibitors Impairs Growth and Early Metabolism in <i>Escherichia coli</i> Through β- and γ-Carbonic Anhydrase-Associated Processes.

International journal of molecular sciences·2026
Same author

Solution Structures and Dynamics of Diamagnetic Lu- and Y-DOTAM/Fluoride Ternary Complexes: The Role of <sup>19</sup>F-CEST in the Detection of the Complex Fluoride Speciation.

Inorganic chemistry·2026
Same author

Green Synthesis of a Hyaluronan-Based Multimeric A9 Peptide System: Polysaccharide-A9 Conjugate with Enhanced HER2 Receptor Binding Affinity and Potential Biomedical Applications as an Active Material.

Biomacromolecules·2025
Same author

Efficacy of a mixture of simethicone and tyndallized bacillus coagulans in infant colic: a pilot study on behalf of Italian Society of Pediatrics (SIP).

Italian journal of pediatrics·2025

Related Experiment Video

Updated: Jun 14, 2026

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors
11:52

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

Published on: June 18, 2013

11.1K

Fluorescence Studies: A9 Peptide, Functionalized with a Fluorogenic Probe, Interacts with Its Receptor Model

Valentina Verdoliva1,2, Giuseppe Digilio3, Ivana Miletto3

  • 1Institute of Biostructures and Bioimaging, National Research Council, 80134 Naples, Italy.

ACS Medicinal Chemistry Letters
|May 19, 2022
PubMed
Summary

Researchers developed a new method to attach a fluorogenic probe to peptide A9 for studying biomolecular interactions. This functionalized peptide enables deeper investigation into the binding processes between peptide ligands and receptor fragments.

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.4K
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
10:41

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Published on: February 17, 2017

8.1K

Related Experiment Videos

Last Updated: Jun 14, 2026

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors
11:52

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

Published on: June 18, 2013

11.1K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.4K
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
10:41

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Published on: February 17, 2017

8.1K

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Chemical Biology

Background:

  • Studying biomolecular interactions is crucial for understanding biological processes.
  • Functionalizing peptides with probes can facilitate interaction studies.
  • Previous methods may lack efficiency or specificity.

Purpose of the Study:

  • To develop a novel synthetic protocol for functionalizing peptide A9 with a fluorogenic probe.
  • To enable detailed investigation of peptide-ligand/receptor-fragment interactions.
  • To elucidate structural determinants involved in the binding process.

Main Methods:

  • Synthetic protocol involving nucleophilic attack on a halo-substituted benzofurazan by a cysteine sulfhydryl group.
  • Utilized basic catalysis with activated zeolites (4 Å molecular sieves).
  • Employed microwave irradiation to promote the reaction.

Main Results:

  • Successfully synthesized a fluorogenically functionalized peptide A9.
  • Introduced a donor-acceptor pair within the peptide sequence, enabling fluorescence studies.
  • Demonstrated the utility of the probe for investigating peptide-receptor interactions.

Conclusions:

  • The developed synthetic protocol is effective for creating functionalized peptides for interaction studies.
  • The fluorogenic probe provides new insights into the structural determinants of peptide-receptor binding.
  • This method advances the study of biomolecular interactions.