Chromosorb 101 as a packing material for reversed-phase chromatography.

Area of Science:

• Analytical Chemistry
• Chromatography

Background:

• Gas chromatographic adsorbents are typically not used in liquid chromatography.
• Exploring alternative packing materials for high-performance liquid chromatography (HPLC) is crucial for method development.

Purpose of the Study:

• To describe the preparation of a Chromosorb 101 column for liquid chromatography.
• To evaluate the performance of Chromosorb 101 as a stationary phase in HPLC.
• To compare the chromatographic behavior of Chromosorb 101 with established HPLC phases.

Main Methods:

• Preparation of a Chromosorb 101 packed column.
• Calculation of column parameters: permeability, efficiency, and peak asymmetry factor.
• Elution of benzene and phenol derivatives using various mobile phases.

Main Results:

• Successful preparation of a Chromosorb 101 liquid chromatographic column.
• Quantification of key column performance metrics.
• Demonstrated elution of aromatic compounds, indicating suitability for separation tasks.
• Observed chromatographic behavior analogous to C18 silica bonded phases.

Conclusions:

• Chromosorb 101, a gas chromatography adsorbent, is a viable packing material for HPLC columns.
• Styrene copolymer packing exhibits properties comparable to C18 silica phases.
• This finding offers a cost-effective alternative for certain HPLC applications.