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Updated: Sep 22, 2025
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Published on: November 16, 2018
[Differential expression of pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia]
Xin-Hai Lin1, Hao-Dong Wang, Long Jiang
1Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Purpose:
To explore pro-angiogenic miRNAs differentially expressed in dental tissue-derived stem cells under hypoxia.
Methods:
Stem cells from apical papilla, dental pulp stem cells and periodontal ligament stem cells were obtained from 3 patients, and treated with nomoxia or hypoxia for 1, 3, 5 d, respectively. Real-time PCR was performed to evaluate the expression of 9 specifically identified pro-angiogenic miRNAs and HIF-1α. SPSS 19.0 software package was used for statistical analysis.
Results:
At the fifth day of hypoxic condition, as for SCAPs, the expression of miR-126 was relatively increased while miR-20a, miR-20b, miR-21, miR-130a, miR-132, miR-210 and miR-503 deceased. As for DPSCs, the expression of miR-21, miR-130a, miR-126 and miR-210 were relatively increased while miR-132 decreased. As for PDLSCs, the expression of miR-126, miR-21 and miR-296 were relatively increased. Finally, the expression of HIF-1α was relatively upregulated in all three type of cells.
Conclusions:
Pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia exists specific expression profile.
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