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Updated: Sep 22, 2025
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
[Protection effect of baicalin-regulated IKKα on inflammatory reaction of human oral keratinocytes]
Zhuan-Zhuan Liu1, Na Xu, Rui-Fang Gao
1School of Stomatology, Shanxi Medical University, The Key Laboratory of Oral Disease Prevention and New Materials of Shanxi Province. Taiyuan 030001, Shanxi Province, China.
Purpose:
To observe the regulation of baicalin on IKKα mediated MASPIN in Human oral keratinocytes (HOKs) inflammatory reaction, this study was to explore the molecular regulation mechanism of baicalin on oral mucosal inflammation.
Methods:
HOKs were stimulated by lipopolysaccharide (LPS) to mimic the inflammatory response of oral mucosal inflammation in vitro. CCK-8 assay was used to detect the toxicity of baicalin to HOKs; then different concentrations of baicalin were pre-treated to LPS-stimulated HOKs, enzyme-linked immunosorbent assays (ELISA) was used to detect the secretion of IL-6 and TNF-α in LPS-stimulated HOKs; reverse transcription polymerase chain reaction(RT-PCR) and Western blot assay were used to detect the regulatory effects of baicalin on gene and protein expression levels of IKKα mediated MASPIN in LPS-stimulated HOKs. SPSS 21.0 software package was used for statistical analysis of the data.
Results:
HOKs stimulated by 10 μg/mL LPS successfully simulated the inflammatory environment of oral mucosal inflammation. The concentration of baicalin between 1 μg/mL and 20 μg/mL had no toxic effect on HOKs. With the increasing concentration of baicalin, the expression of MASPIN increased gradually, while the expression of IKKα and inflammatory factors decreased gradually(P＜0.05).
Conclusions:
Baicalin can decrease the expression of inflammatory factors in LPS-stimulated HOKs, down-regulate IKKα and up-regulate MASPIN.
