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A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications.

Lars-Erik Meyer1, Mads Bruno Andersen1, Selin Kara1,2

  • 1Department of Biological and Chemical Engineering, Biocatalysis and Bioprocessing Group, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus, Denmark.

Angewandte Chemie (International Ed. in English)
|May 19, 2022
PubMed
Summary
This summary is machine-generated.

This study explores a thermomorphic multiphasic system (TMS) for biocatalysis, using a deep eutectic solvent (DES) and a buffer. The system efficiently separates enzyme and substrate, enabling catalyst recycling.

Keywords:
Aqueous Two-Phase SystemsBiocatalysisDeep Eutectic SolventsEnzymesThermomorphic Multiphasic Systems

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Area of Science:

  • Biocatalysis
  • Green Chemistry
  • Separation Science

Background:

  • Homogeneous biocatalysis often faces challenges in enzyme and product separation.
  • Thermomorphic multiphasic systems (TMS) offer a potential solution by utilizing phase transitions for separation.

Purpose of the Study:

  • To investigate the applicability of a novel thermomorphic multiphasic system (TMS) for homogeneous biocatalysis.
  • To evaluate a lidocaine-based deep eutectic solvent (DES) combined with an aqueous buffer for enzyme-catalyzed reactions.

Main Methods:

  • Determination of phase diagrams for the TMS composed of a hydrophobic DES and an aqueous potassium phosphate buffer.
  • Investigation of the effect of additional components on the system's cloud point.
  • Distribution studies to analyze the partitioning of enzyme, substrate, and product.
  • Performance evaluation of a horse liver alcohol dehydrogenase-catalyzed reduction using the TMS.

Main Results:

  • The TMS exhibited a lower critical solution temperature (LCST) phase change.
  • Addition of components decreased the cloud point by approximately 0.026°C per concentration unit.
  • Proteins remained in the aqueous buffer phase (>95%), while hydrophobic substrates/products concentrated in the DES phase (>95%).
  • Successful horse liver alcohol dehydrogenase-catalyzed reduction was achieved, with the biocatalyst recycled for three runs.

Conclusions:

  • The investigated thermomorphic multiphasic system (TMS) is effective for homogeneous biocatalysis.
  • The system facilitates efficient separation of enzymes from hydrophobic substrates and products.
  • The biocatalyst demonstrated recyclability, highlighting the system's potential for sustainable chemical processes.