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Pervasive transcription: a controlled risk.

Tommaso Villa1, Odil Porrua1

  • 1Institut Jacques Monod, CNRS, Université de Paris Cité, France.

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|May 19, 2022
PubMed
Summary

Eukaryotic genomes produce many noncoding RNAs (ncRNAs) from accessible DNA. Budding yeast models how cells control ncRNA production to prevent cellular dysfunction.

Keywords:
RNA degradationRNA quality controlnoncoding RNAspervasive transcriptiontranscription termination

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Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Eukaryotic genomes exhibit pervasive transcription by RNA polymerase II.
  • Accessible chromatin regions can be transcribed, leading to abundant noncoding RNA (ncRNA) production.
  • ncRNAs can potentially interfere with gene expression regulation.

Purpose of the Study:

  • To review the mechanisms and strategies used by budding yeast to control pervasive transcription.
  • To discuss the risks associated with losing control over ncRNA production.
  • To highlight the importance of ncRNA regulation in cellular function.

Main Methods:

  • Review of existing literature on pervasive transcription in budding yeast.
  • Analysis of molecular actors and regulatory strategies.
  • Discussion of experimental findings on ncRNA control.

Main Results:

  • Budding yeast employs specific actors and strategies to govern ncRNA production.
  • Dysregulation of pervasive transcription can lead to hazardous disruption of cellular functions.
  • Understanding yeast models provides insights into conserved eukaryotic mechanisms.

Conclusions:

  • Pervasive transcription is a fundamental aspect of eukaryotic genome activity.
  • Effective control of ncRNA production is crucial for maintaining cellular homeostasis.
  • Budding yeast serves as a valuable model for studying ncRNA regulation and its implications.