Solid phase enzyme immunoassays of pertussis toxin using peroxidase or biotin labelled antibodies

The quantitative determination of pertussis toxin (PT) is generally estimated by biological tests which are time-consuming, cumbersome and unsuitable for simultaneous testing of a large number of samples. The present work describes a rapid and sensitive ELISA procedure allowing PT assay based on a sandwich technique amplified via avidin-biotin interaction. As low as 0.1 ng of PT in 0.1 ml sample could be detected by the procedure described.