Growth Rates of Hydrogen Microbubbles in Reacting Femtoliter Droplets

Chemical reactions in small droplets are extensively explored to accelerate the discovery of new materials and increase the efficiency and specificity in catalytic biphasic conversion and high-throughput analytics. In this work, we investigate the local rate of the gas-evolution reaction within femtoliter droplets immobilized on a solid surface. The growth rate of hydrogen microbubbles (≥500 nm in radius) produced from the reaction was measured online with high-resolution confocal microscopic images. The growth rate of bubbles was faster in smaller droplets and near the droplet rim in the same droplet. The results were consistent for both pure and binary reacting droplets and on substrates of different wettability. Our theoretical analysis based on diffusion, chemical reaction, and bubble growth predicted that the concentration of the reactant depended on the droplet size and the bubble location inside the droplet, in good agreement with experimental results. Our results reveal that the reaction rate may be spatially nonuniform in the reacting microdroplets. The findings may have implications for formulating the chemical properties and uses of these droplets.