Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.0K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.0K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

2.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.4K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.8K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.8K
Types of Membrane Protrusions01:28

Types of Membrane Protrusions

3.0K
The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most  widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections...
3.0K
Cell Migration01:09

Cell Migration

17.3K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
17.3K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.5K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Outcome of Replanted Avulsed Anterior Teeth: A Retrospective Cohort Study.

Journal of endodontics·2026
Same author

Effects of repeated preheating on polymerization shrinkage kinetics and Vickers microhardness of resin composites: an in vitro study.

BMC oral health·2026
Same author

Diode-pumped prism-free Ti:sapphire oscillator delivering 10-fs pulses.

Optics express·2026
Same author

Prediction of delirium in trauma patients using interpretable machine learning.

Scientific reports·2026
Same author

Characteristics of Cardiovascular Disease Prediction Models Considering Mental Disorders: A Systematic Review.

Journal of the American Heart Association·2026
Same author

Development of an Artificial Intelligence Model to Predict Endotracheal Intubation in Critically Ill Patients in Real Time.

Journal of clinical medicine·2026
Same journal

A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

The Journal of cell biology·2026
Same journal

Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

The Journal of cell biology·2026
Same journal

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026
Same journal

Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

The Journal of cell biology·2026
Same journal

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same journal

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026
See all related articles

Related Experiment Video

Updated: Sep 22, 2025

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

2.5K

Crumbs complex-directed apical membrane dynamics in epithelial cell ingression.

Sérgio Simões1, Gerald Lerchbaumer1, Milena Pellikka1

  • 1Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada.

The Journal of Cell Biology
|May 19, 2022
PubMed
Summary
This summary is machine-generated.

The Crumbs polarity complex regulates neural stem cell ingression by controlling apical constriction and domain removal. This process integrates cell fate, polarity, and actomyosin contractility for development and cancer.

More Related Videos

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

5.5K
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy

Published on: October 3, 2017

13.6K

Related Experiment Videos

Last Updated: Sep 22, 2025

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

2.5K
Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

5.5K
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy

Published on: October 3, 2017

13.6K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Cancer Biology

Background:

  • Epithelial cells ingress during development and cancer, losing apical membranes and junctions.
  • The role of apical-basal polarity factors in cell ingression remains unclear.

Purpose of the Study:

  • To investigate the role of the Crumbs polarity complex in neural stem cell ingression.
  • To elucidate the molecular mechanisms governing cell ingression dynamics.

Main Methods:

  • Studied neural stem cell ingression dynamics.
  • Investigated the regulation of the Crumbs polarity complex, including endocytosis and recycling.
  • Analyzed the roles of Crumbs, Cysts, and Neuralized in ingression.

Main Results:

  • Dynamic regulation of the Crumbs complex is essential for neural stem cell ingression.
  • Crumbs endocytosis and recycling control the timing of ingression.
  • Crumbs and Cysts promote apical constriction, while Neuralized mediates apical domain removal.

Conclusions:

  • The Crumbs complex integrates cell fate, polarity, endocytosis, and contractility for cell ingression.
  • This mechanism is fundamental to morphogenetic processes in development and cancer.