Rotative agitation RIA. A high sensitivity technique for hepatitis B surface antigen (HBsAg) determinations

With the objective of finding a simpler and more sensitive method than conventional radioimmunoassay testing to detect HBsAg, we have modified the sample volume and first incubation procedure of the Ausria IIA method. There was an up to 9-fold increase in sensitivity without loss of specificity, in different assays, when the first incubation was carried out with a serum sample of 1 ml that was rotatively agitated for 22 h at room temperature. Additionally, 71 samples from which conventional testing had isolated hepatitis B core antibodies were tested with the modified rotative agitation technique and 9 of these specimens (12.67%) were positive for HBsAg. Routine employment of the rotative agitation radioimmunoassay technique to screen HBsAg by blood banks, to detect potential infectious donations that escape conventional testing, could be an effective as well as low-cost procedure to prevent post-transfusion hepatitis B.