Related Experiment Video

Updated: Sep 22, 2025

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
11:00

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

Published on: March 21, 2021

4.4K

Vitamin E protective effects on genomic and cellular damage caused by paediatric preventive supplementation for

Rocío Celeste Gambaro1, Analía Seoane1, Gisel Padula1,2

  • 1IGEVET, Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata1900, Argentina.

The British Journal of Nutrition
|May 20, 2022
PubMed

Insights

Daily iron supplementation may harm cell viability and increase oxidative stress, unlike weekly iron. Vitamin E may mitigate these adverse effects, offering a safer approach to preventing iron deficiency anaemia.

Area of Science:

  • Biochemistry
  • Hematology
  • Cell Biology

Background:

  • Iron deficiency is a primary cause of anemia, with high prevalence in Argentina.
  • Current pediatric recommendations include daily ferrous sulfate supplementation for anemia prevention.
  • Potential risks of excess iron, such as oxidative stress and cytomolecular damage, are a concern.

Purpose of the Study:

  • To evaluate the in vitro effects of daily versus weekly ferrous sulfate supplementation combined with vitamin E.
  • To assess impacts on cell viability, oxidative stress, and genomic damage in peripheral blood cultures.

Main Methods:

  • In vitro culture of peripheral blood cells.
  • Experimental groups included controls, vitamin E alone, weekly ferrous sulfate with vitamin E, and daily ferrous sulfate with vitamin E.
  • Measurements included cell viability, reactive oxygen species, lipid peroxidation, and cytomolecular damage.

Main Results:

  • Daily ferrous sulfate significantly decreased cell viability and increased oxidative stress markers compared to weekly supplementation.
  • Excess iron from daily supplementation was linked to increased reactive oxygen species, lipid peroxidation, and genomic damage.
  • Vitamin E demonstrated a potential to reduce ferrous sulfate-induced oxidative stress and DNA damage.

Conclusions:

  • Daily ferrous sulfate supplementation poses risks of reduced cell viability and increased oxidative/genomic damage.
  • Weekly ferrous sulfate supplementation appears safer and effective for preventing anemia.
  • Vitamin E may serve as a protective agent against iron-induced cellular damage.

Keywords:
Cytomolecular damageIron deficiency anaemiaOxidative stressPreventive treatmentα-tocopherol

More Related Videos

Measurement of Heme Synthesis Levels in Mammalian Cells09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

12.2K
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System08:48

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Published on: December 21, 2016

8.8K

Related Concept Videos

Vitamins01:30

Vitamins

Vitamins, derived from the Latin word for life, are essential organic substances required in small quantities for optimal growth and overall well-being. Unlike other organic nutrients, vitamins don't act as sources of energy or building materials but rather facilitate these nutrients' utilization by the body. Vitamins are predominantly coenzymes, assisting enzymes in specific chemical actions, like the oxidation of glucose for energy involving B vitamins. Most vitamins are not produced...
1.0K
Teratogenicity01:07

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
2.8K
Factors Affecting Erythropoiesis01:24

Factors Affecting Erythropoiesis

The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
3.7K