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Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
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Using Light-Sheet Microscopy to Study Spontaneous Activity in the Developing Lateral-Line System.

Qiuxiang Zhang1, Katie S Kindt1

  • 1Section on Sensory Cell Development and Function, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD, United States.

Frontiers in Cell and Developmental Biology
|May 20, 2022
PubMed
Summary
This summary is machine-generated.

Spontaneous activity in zebrafish lateral-line hair cells originates in the mechanosensory bundle and presynapse. This autonomous activity in hair cells drives calcium influx, independent of supporting cells or efferent terminals.

Keywords:
developmenthair celllateral line efferentslight-sheet microscopyspontaneous activity

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Sensory Biology

Background:

  • Hair cells are crucial sensory receptors in vertebrate auditory, vestibular, and aquatic lateral-line systems.
  • Understanding spontaneous activity in these cells is vital for sensory system development and function.

Purpose of the Study:

  • To investigate spontaneous activity in the developing zebrafish lateral-line system in vivo.
  • To identify the cellular origins and mechanisms of spontaneous activity in hair cells.

Main Methods:

  • Utilized genetically encoded calcium indicators and light-sheet fluorescence microscopy.
  • Performed two-color functional imaging to analyze correlated activity.
  • Employed localized calcium indicators to pinpoint activity domains within hair cells.

Main Results:

  • Confirmed spontaneous calcium activity in developing lateral-line hair cells.
  • Demonstrated spontaneous activity in surrounding supporting cells and cholinergic efferent terminals.
  • Showed hair cell activity is autonomous and does not correlate with supporting cells or efferent terminals.
  • Identified spontaneous activity in hair cells occurring in the mechanosensory bundle and presynapse.
  • Established that mechanosensory bundle activity drives presynaptic calcium influx.

Conclusions:

  • Developing lateral-line hair cells autonomously generate spontaneous activity.
  • Spontaneous activity originates from mechanosensory events within hair cells.
  • This intrinsic activity is fundamental to hair cell development and function.