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Updated: Sep 22, 2025

A Colorimetric Method for Measuring Iron Content in Plants
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A Quick Method to Quantify Iron in Arabidopsis Seedlings.

Chandan Kumar Gautam1, Huei-Hsuan Tsai1, Wolfgang Schmidt1,2,3

  • 1Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.

Bio-Protocol
|May 20, 2022
PubMed
Summary
This summary is machine-generated.

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This study presents a simple, cost-effective spectroscopy method for quantifying iron (Fe) in Arabidopsis plants. This approach avoids expensive equipment, making plant iron analysis more accessible for researchers.

Area of Science:

  • Plant Physiology
  • Plant Molecular Biology
  • Biochemistry

Background:

  • Iron (Fe) is a vital micronutrient essential for plant growth and development.
  • Maintaining optimal iron levels is crucial, as both deficiency and excess are detrimental to plant health.
  • Accurate quantification of iron in plant tissues is important for assessing plant fitness, genetic variations, and gene function.

Purpose of the Study:

  • To develop a simple, cost-efficient, and accessible protocol for quantifying iron concentrations in plant samples.
  • To provide an alternative to expensive and complex methods like ICP-OES and ICP-MS for iron determination.
  • To enable precise measurement of iron levels in Arabidopsis roots and shoots.

Main Methods:

  • A spectroscopy-based assay was developed to quantify iron (Fe2+) - BPDS3 complexes.
Keywords:
ArabidopsisBPDSIron quantificationPlant NutritionSpectrophotometry

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  • The protocol utilizes a simple colorimetric reaction with a set of standards for calibration.
  • Measurements were performed on roots and shoots of Arabidopsis seedlings.

    • Main Results:

    • The developed protocol provides a fast and reproducible method for determining iron levels.
    • The assay achieves high precision in quantifying iron concentrations.
    • The method is cost-efficient and does not require sophisticated or expensive equipment.

    Conclusions:

    • This spectroscopy-based assay offers a practical and accessible solution for quantifying iron in plant samples.
    • The protocol is suitable for researchers lacking access to advanced analytical instrumentation.
    • It enables precise and reliable assessment of iron status in Arabidopsis, facilitating plant research.