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Related Experiment Videos

[A simple and rapid crystal violet uptake sensitivity test for anticancer agents].

T Fujii, M Maeda, Y Kawashima

    Nihon Sanka Fujinka Gakkai Zasshi
    |March 1, 1987
    PubMed
    Summary

    A new in vitro micromethod accurately predicts anticancer drug effectiveness for individual cancer patients. This rapid and reproducible assay improves chemotherapy success rates by enabling personalized drug selection.

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    [Psychosocial approach of the epileptic patient: analysis of 30 adults].

    Arquivos de neuro-psiquiatria·1992
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    Manganese-containing superoxide dismutase in blood and urine during open-heart surgery.

    Japanese circulation journal·1992
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    [A case of colon angiodysplasia successfully treated with endoscopy].

    Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·1992
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    Occurrence of intraportally-infused urea-15N in the urine of domestic fowl.

    British poultry science·1992
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    Escherichia coli F0F1-ATPase. Residues involved in catalysis and coupling.

    Annals of the New York Academy of Sciences·1992
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    Studies on anticoagulant-active arabinan sulfates from the green alga, Codium latum.

    Carbohydrate research·1992

    Area of Science:

    • Oncology
    • Biochemistry
    • Cell Biology

    Context:

    • Traditional anticancer drug selection relies on clinical experience.
    • Effective chemotherapy requires personalized drug selection for individual cancer patients.
    • Current in vitro methods have limitations in speed, cell quantity, or reproducibility.

    Purpose:

    • To introduce a novel, rapid, and reproducible in vitro micromethod for quantifying cancer cell cytotoxicity.
    • To assess the chemosensitivity of gynecologic malignancies using the developed micromethod.
    • To establish a reliable method for selecting effective anticancer drugs for individual patients.

    Summary:

    • A microplate-based assay involves incubating tumor cells with anticancer drugs for 48 hours.
    • Cytotoxicity is quantified by measuring absorbance after staining surviving cells with crystal violet.

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  • The method is simple, rapid, requires minimal cells, and correlates well with isotope-based sensitivity tests.

    • Impact:

    • The developed micromethod demonstrated a 77% success rate in chemosensitivity testing for gynecologic malignancies.
    • This assay offers higher success rates compared to existing in vitro methods.
    • The method is poised for widespread adoption in various cancer treatment fields.