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Updated: Sep 22, 2025
Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells
Published on: May 12, 2023
Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons
Yuxiao Mei1, Zhichao Liu1, Meijun Liu1
1Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai 200241, China. ytian@chem.ecnu.edu.cn.
Abstract:
Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca2+ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.
