Polymorphism of the leptin gene in buffalo breed groups from eastern Amazon
Lorena Keyse Silva1, Elizabeth Machado Barbosa2, Sandra Cristina Becker Silva3
1Universidade Federal Rural da Amazônia. lorenakeyse24@gmail.com.
Veterinaria Italiana
|May 20, 2022
Summary
Genetic characterization of buffalo herds in Varzea and Terra-Firme ecosystems revealed distinct leptin gene (LEP-1620) polymorphisms. Murrah TF buffaloes showed the highest allele A frequency, while Mediterranean TF had the lowest.
Area of Science:
- Animal Genetics
- Molecular Biology
- Ecosystem Adaptation
Background:
- Buffalo farming is crucial in various ecosystems.
- Understanding genetic diversity aids in breed improvement and conservation.
- The leptin gene (LEP-1620) plays a role in animal metabolism and growth.
Purpose of the Study:
- To genetically characterize buffalo herds from Varzea (VA) and Terra-Firme (TF) ecosystems.
- To analyze the polymorphism of intron 2 of the leptin gene (LEP-1620).
- To assess genetic diversity and population structure in different environmental conditions.
Main Methods:
- PCR-RFLP method was used to analyze LEP-1620 polymorphism (SNP).
- Two hundred seventy-nine buffaloes from four populations were evaluated.
- Restriction enzyme BsaAI was employed for genotyping.
Main Results:
- Two alleles (A and G) were detected, with allele A frequencies varying significantly between populations.
- Murrah TF buffaloes exhibited the highest allele A frequency (0.850), while Mediterranean TF had the lowest (0.395).
- The Crossbred VA herd showed significant inbreeding, unlike other populations.
Conclusions:
- LEP-1620 polymorphism differs across buffalo populations and ecosystems.
- Genetic diversity indices suggest varying population structures.
- Findings provide insights into buffalo adaptation and genetic management strategies for different environments.
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