Related Experiment Video
Updated: Sep 22, 2025
08:33
An Ex vivo Culture System to Study Thyroid Development
Published on: June 6, 2014
11.7K
Differential gene expression profile of multinodular goiter
Wenberger Lanza Daniel de Figueiredo1, Eraldo Ferreira Lopes2, Deborah Laredo Jezini3
1College of Medicine, Nilton Lins University, Manaus, Amazonas, Brazil.
Plos One
|May 20, 2022
Summary
Multinodular goiter exhibits a pro-tumoral gene expression profile. The HOTS gene is uniquely expressed in goiter tissues, suggesting its central role in disease development.
Area of Science:
- Molecular Biology
- Genomics
- Endocrinology
Background:
- Multinodular goiter is a significant cause of thyroidectomies in endemic areas.
- It is a heterogeneous molecular disease with complex underlying mechanisms.
Purpose of the Study:
- To analyze differential gene expression in multinodular goiter tissues.
- To compare gene expression profiles with non-goiter thyroid tissue.
- To identify potential molecular markers involved in goiter development.
Main Methods:
- RNA sequencing (RNA-seq) technology was employed.
- Illumina HiSeq 2000 platform and NEBNext® Ultra™ RNA Library Prep Kit were used.
- Differential gene expression analysis was performed on surgical specimens.
Main Results:
- Significant differences in gene expression were observed between multinodular goiter and healthy thyroid tissues.
- At least 70 genes showed differential expression.
- The HOTS gene was found to be exclusively expressed in multinodular goiter tissues (p < 0.05).
Conclusions:
- Multinodular goiter displays a pro-tumoral gene expression signature.
- The HOTS gene may play a critical role in the pathogenesis of multinodular goiter.
Related Concept Videos
01:20Synthesis and Regulation of Thyroid Hormones
5.2K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
5.2K
01:23The Thyroid Gland
4.4K
The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
4.4K
02:55The Nucleolus
9.2K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
9.2K
01:58Cell Specific Gene Expression
14.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.1K