Related Experiment Video
Updated: Sep 22, 2025
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Selection, truncation and fluorescence polarization based aptasensor for Weissella viridescens detection.
Pengfei Ma1, Nuo Duan1, Hua Ye2
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology Jiangnan University, Wuxi, 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China.
Researchers developed a novel aptamer to detect Weissella viridescens, a spoilage bacterium in meat. A fluorescence polarization platform using this aptamer offers a rapid and sensitive method for detecting bacteria in food products.
Area of Science:
- Food Microbiology
- Biotechnology
- Biosensing
Background:
- Weissella viridescens is a common spoilage bacterium in low-temperature meat products.
- Accurate and rapid detection of W. viridescens is crucial for food safety and quality control.
Purpose of the Study:
- To develop a novel aptamer for specific recognition of Weissella viridescens.
- To construct a rapid and sensitive biosensing platform for W. viridescens detection.
Main Methods:
- Whole-cell systemic evolution of ligands by exponential enrichment (SELEX) was used to select aptamers.
- Aptamer optimization through truncation and a fluorescence polarization (FP) platform were employed for detection.
- Streptavidin and a FAM-labeled complementary sequence were used for signal amplification and generation.
Main Results:
- A novel aptamer L3 with a Kd of 68.25 nM and an optimized aptamer TL43 with a Kd of 32.11 nM were obtained.
- A fluorescence polarization platform demonstrated a linear detection range from 102 to 106 cfu/mL for W. viridescens.
- The detection system showed a good recovery rate (90.6%-107.7%) in smoked ham samples.
Conclusions:
- The selected and truncated aptamer TL43 is highly specific for W. viridescens.
- The developed fluorescence polarization biosensing platform is rapid, sensitive, and suitable for practical applications in food safety.
More Related Videos
12:31A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
07:10Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025