Effects of environmental factor fulvic acid on AgNPs food chain delivery and bioavailability

Due to its antimicrobial activity, silver nanoparticles (AgNPs) have become the most commonly applied nanomaterials. However, the potential ecotoxicological toxicity of AgNPs in the environment is still unclear. Here we assessed the trophic transfer and toxicity of commercially manufactured polyvinyl pyrrolidone (PVP)-coated AgNPs using a model food chain from Escherichia coli (E. coli) to Caenorhabditis elegans (C. elegans). Our results demonstrated that AgNPs could be accumulated in E. coli and transferred to C. elegans that preyed on the bacteria. Although low concentration of AgNPs had no significant inhibition on E. coli, they could affect germ cell apoptosis, reproduction ability and population size of C. elegans through food chain. Importantly, natural organic matter (NOM), which is omnipresent in environmental system, could increase the accumulation of AgNPs in E. coli and C. elegans, and significantly enhance the ecotoxicity of AgNPs. Our findings indicated that potential risks of nanomaterial through food chain should be considered for higher trophic organisms. And environmental factors could play an important role in transport of nanomaterials and altering their accumulation and toxicity in ecosystem.