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Related Concept Videos

In Vitro Fertilization01:24

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In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
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Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
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Related Experiment Video

Updated: Sep 22, 2025

Human Blastocyst Biopsy and Vitrification
10:59

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Is there an optimal timing interval between hCG trigger and oocyte vitrification?

Yu-Wen Weng1, Ming-Huei Lin2, Robert Kuo-Kuang Lee3

  • 1Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei, Taiwan.

Taiwanese Journal of Obstetrics & Gynecology
|May 20, 2022
PubMed
Summary

Optimal timing for oocyte vitrification is not critical for survival rates. This study found no correlation between the time intervals after human chorionic gonadotropin (hCG) trigger and oocyte equilibration time with oocyte survival rates during vitrification.

Keywords:
CryopreservationOocyteSurvival rateVitrificationhCG trigger

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Area of Science:

  • Reproductive Medicine
  • Cryobiology
  • In Vitro Fertilization

Background:

  • Oocyte vitrification is a key fertility preservation technique.
  • Clinical success in oocyte vitrification is operator-dependent.
  • Optimal timing for cryoprotectant exposure and thawing is crucial.

Purpose of the Study:

  • To determine the optimal time intervals between human chorionic gonadotropin (hCG) trigger and oocyte vitrification.
  • To evaluate the impact of oocyte equilibration time on vitrification success.
  • To identify critical time windows for improved oocyte cryopreservation outcomes.

Main Methods:

  • Retrospective analysis of 66 patients undergoing vitrified-thawed oocyte cycles.
  • Oocytes grouped by intervals: hCG trigger to vitrification (<38h, 38-39h, >39h, in vitro maturation [IVM]).
  • Oocyte equilibration times categorized (<10 min, 10-12 min, 12-15 min).

Main Results:

  • No significant difference in oocyte survival rates across hCG trigger intervals (97.59% to 100%).
  • A trend of decreased survival was observed in the IVM group (66.67%).
  • Oocyte survival rates were consistent across equilibration times (96.77% to 97.33%).

Conclusions:

  • Oocyte survival rates are not correlated with time intervals between hCG trigger and vitrification.
  • Equilibration time during vitrification does not significantly affect survival rates for mature oocytes.
  • Further prospective studies are needed to confirm clinical outcomes.