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Modification-Free Fluorescent Biosensor for CEA Based on Polydopamine-Coated Upconversion Nanoparticles.

Dezhong Yu1,2, Zhonghui Zha1,2, Sheng Tang1,2

  • 1School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan, 430205, People's Republic of China.

Journal of Fluorescence
|May 21, 2022
PubMed
Summary
This summary is machine-generated.

A novel, modification-free biosensor using polydopamine-coated upconversion nanoparticles (UCNPs@PDA) and aptamer-functionalized gold nanoparticles (AuNPs) offers sensitive detection of cancer embryonic antigen (CEA). This advancement simplifies protein sensing and shows great potential for clinical diagnostics.

Keywords:
AptamerBiosensorFluorescencePolydopamineUpconversion nanoparticles

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Upconversion nanoparticles (UCNPs) are successful in in vitro protein sensing.
  • Aptamer modification of UCNPs is common but faces challenges like complex DNA modification and phosphate group adsorption.
  • Existing methods limit the practical application of UCNP-based biosensors.

Purpose of the Study:

  • To develop a modification-free fluorescent biosensor for sensitive protein detection.
  • To overcome limitations associated with UCNP modification for protein assays.
  • To create a robust biosensor for cancer embryonic antigen (CEA) quantification.

Main Methods:

  • A polydopamine-coated UCNP (UCNPs@PDA) and CEA aptamer-functionalized AuNP (AuNPs-CEA aptamer) system was designed.
  • Fluorescence resonance energy transfer (FRET) between UCNPs@PDA and AuNPs-CEA aptamer was utilized.
  • The displacement of AuNPs-CEA aptamer by CEA triggered fluorescence recovery for quantification.

Main Results:

  • The biosensor demonstrated sensitive CEA detection with a linear range of 0.1–100 ng/mL in aqueous solution.
  • A low limit of detection (LOD) of 0.031 ng/mL was achieved in aqueous solution.
  • The biosensor maintained sensitivity in human serum samples, with an LOD of 0.055 ng/mL.

Conclusions:

  • The proposed modification-free biosensor simplifies UCNP modification for protein assays.
  • The UCNPs@PDA and AuNPs-CEA aptamer system offers a sensitive and practical approach for CEA detection.
  • This biosensor shows significant potential for application in clinical diagnostics and protein sensing.