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Updated: Sep 22, 2025
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
The complex hydrogel based on diatom biosilica and hydroxybutyl chitosan for wound healing
Hussein E Rozan1, Guangsheng Wu2, Zhongzheng Zhou3
1College of Marine Life Science, Ocean University of China, Qingdao 266003, China; Department of Biochemistry, Faculty of Agriculture, Al-Azhar University, Nasr City 11751, Cairo, Egypt.
Abstract:
In this study, doxycycline (DOXY)-loaded diatom biosilica (DBs) were developed and coated with hydroxybutyl chitosan (HBC) hydrogel for wound healing. The HBC/DBs/DOXY composite hydrogel had significant inhibitory activity against S. aureus (100%) and E. coli (98%). In addition, the HBC/DBs/DOXY hydrogel showed minimum cytotoxicity on L929 cells in vitro, indicating the great biocompatibility of the composite hydrogel. The in vivo results demonstrated that HBC/DBs/DOXY composite hydrogel could promote the wound re-epithelialization and accelerate the healing. The wound closure was evaluated to be 99.4 ± 0.4% at day 12 after treated with the hydrogel, with the presence of neovascularization and collagen deposition, all indicating the great potential of HBC/DBs/DOXY hydrogel in wound healing.
