Assessment of a potential bioproduct for controlling Cerotoma arcuata tingomariana (coleoptera: Chrysomelidae)

The leaf-feeding pest Cerotoma arcuata tingomariana (Bechyné) (Coleoptera: Chrysomelidae) produces huge economic losses in different crops. This study aimed to produce conidia by semisolid-state fermentation and to establish the insecticidal activity of two formulation prototypes based on a native Beauveria bassiana isolate for controlling this pest.

A novel fabric-based semisolid-state fermentation strategy for quick and large-scale conidia production was performed and characterized. Conidia were formulated as an emulsifiable concentrate (EC) and a water-dispersible granulate (WG). Afterwards, the mortality of C. a. tingomariana adults was assessed. A conidia concentration of 2.9 × 10⁹ conidia cm^-2 was obtained after 9 days-course fermentation and a yield of 33.4 g kg^-1 dry-substrate.

The polyester fabric-based fermentation is an efficient technique for producing and collecting B. bassiana spores. Regarding LC₉₀ , the potency analysis showed that the EC was 21-fold more potent than the non-formulated conidia, and ~ 2.6-fold more potent than the WG.

A high throughput fermentation based on polyester fabric as support for B. bassiana conidia production and subsequent formulation as an EC comprises a promising strategy for obtaining a bioproduct to control adults of C. a. tingomariana and other Chrysomelidae pests.