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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Characterization and complete genome analysis of a novel Escherichia phage, vB_EcoM-RPN242

Napakhwan Imklin1, Pattaraporn Sriprasong1, Narut Thanantong2

  • 1Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom, 73000, Thailand.

Archives of Virology
|May 22, 2022
PubMed

Insights

A novel Escherichia phage, vB_EcoM-RPN242, was discovered from a piglet E. coli strain. This phage exhibits broad temperature and pH stability and lacks virulence genes, suggesting potential as a therapeutic agent.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriophage Research

Background:

  • Escherichia coli (E. coli) infections in livestock can cause significant economic losses.
  • Bacteriophages (phages) are viruses that infect bacteria and are being explored as alternatives to antibiotics.

Purpose of the Study:

  • To isolate and characterize a novel Escherichia phage for potential therapeutic applications.
  • To determine the genomic and stability properties of the isolated phage.

Main Methods:

  • Isolation of bacteriophage vB_EcoM-RPN242 using an E. coli strain from a diarrheic piglet.
  • Plaque assays to determine optimal growth temperature.
  • Stability testing across a range of pH and temperatures.
  • Whole-genome sequencing and bioinformatic analysis.

Main Results:

  • Phage vB_EcoM-RPN242 demonstrated plaque formation at 15-45°C.
  • The phage exhibited stability over a wide pH range (4-10) and temperature range (4-70°C).
  • The genome is linear double-stranded DNA (154,840 bp) with 195 protein-encoding genes and 2 tRNAs, lacking virulence, toxin, or antimicrobial resistance genes.

Conclusions:

  • Phage vB_EcoM-RPN242 is a robust phage with a broad stability profile.
  • The absence of virulence and resistance genes suggests its potential for safe application.
  • Phylogenetic analysis indicates vB_EcoM-RPN242 may represent a new species within the Agtrevirus genus.

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