Related Experiment Video
Updated: Sep 22, 2025
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
[Molecular analysis of 23 cases of B subgroup]
Jinhui Xie1, Shuangyu Li, Mengli Xue
1Tianjin Blood Center, Tianjin 300110, China. xie_jinhui@163.com.
Objective:
To explore the molecular reasons of weak expression of B antigen on the red cell.
Methods:
Serological test for blood group was carried out, including red cell and plasma grouping, and anti-A1 and anti-H testing, and confirming weak A or B antigens by adsorption and elution. Exons 1-7 were sequenced directly, and one of them was cloned and sequenced.
Results:
All of the 23 samples showed the weak B antigen by serological method. The alleles of the subgroups were identified by DNA sequencing, including 2 Bel subgroup, 4 B3 subgroup, 14 Bw subgroup, 2 CisAB subgroup and a novel allele. The novel allele showed a nucleotide substitution 662G>A in the exon 7, and the sequence was submitted to Blood Group Antigen Gene Mutation Database, and the novel allele was named Bel10.
Conclusion:
Nucleotide substitution in exon results in blood subgroup, which showed that the antigens were weakened, and Bw phenotype was the most frequently subgroup.
