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Related Concept Videos

Peptide Bonds02:43

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A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
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Peptide Aggregation Induced Immunogenic Rupture (PAIIR).

Gokhan Gunay1, Seren Hamsici1, Gillian A Lang2

  • 1Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, OK, 73069, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 23, 2022
PubMed
Summary
This summary is machine-generated.

A novel peptide adjuvant, [II], triggers immunogenic cell death (ICD) and DAMPs release. This peptide-aggregation induced immunogenic rupture (PAIIR) approach enhances influenza vaccine efficacy by boosting antibody responses.

Keywords:
damage-associated molecular patternsimmunogenic cell deathinfluenza vaccinationpeptide aggregation

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Area of Science:

  • Immunology
  • Vaccinology
  • Biotechnology

Background:

  • Immunogenic cell death (ICD) involves cellular stress leading to damage-associated molecular pattern (DAMPs) release, which enhances immune responses.
  • DAMPs amplify antigen-specific immunity's longevity and effectiveness.
  • Current methods lack controlled induction of ICD for predictable outcomes.

Purpose of the Study:

  • To develop a peptide-based tool for controlled ICD induction and adjuvant application.
  • To evaluate the efficacy of the peptide-aggregation induced immunogenic rupture (PAIIR) approach as a vaccine adjuvant.

Main Methods:

  • A peptide tool, [II], was designed to aggregate within cells, induce membrane damage, and trigger ICD and DAMPs release.
  • An influenza vaccine was formulated by combining [II] with influenza hemagglutinin (HA) subunit antigens.
  • Immune responses were assessed in mice immunized with the vaccine formulation.

Main Results:

  • The [II] peptide induced ICD and DAMPs release across various cell types.
  • [II] significantly increased HA-specific immunoglobulin G1 (IgG1) and IgG2a antibody titers compared to HA-only immunization.
  • The peptide [II] did not elicit antibodies on its own.

Conclusions:

  • The peptide-aggregation induced immunogenic rupture (PAIIR) represents a novel approach for vaccine adjuvant development.
  • PAIIR demonstrates significant potential as an adjuvant to enhance influenza HA vaccination and promote universal protection.