Related Experiment Video

Updated: Sep 22, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K

SLC8A1 antisense RNA 1 suppresses papillary thyroid cancer malignant progression via the FUS RNA binding protein

Yunchao Xin1, Xiaoling Shang1, Xiaoran Sun2

  • 1Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei, China.

Bioengineered
|May 23, 2022
PubMed

Insights

Long non-coding RNA SLC8A1-AS1 is downregulated in papillary thyroid cancer (PTC). Overexpression of SLC8A1-AS1 inhibits PTC cell proliferation, invasion, and migration by stabilizing Numbl mRNA via FUS, suggesting it as a potential therapeutic target.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Papillary thyroid cancer (PTC) is a common endocrine malignancy with significant morbidity and mortality.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.

Purpose of the Study:

  • To investigate the role of lncRNA SLC8A1 antisense RNA 1 (SLC8A1-AS1) in the pathogenesis of PTC.
  • To elucidate the underlying molecular mechanisms by which SLC8A1-AS1 affects PTC progression.

Main Methods:

  • Quantitative real-time PCR to assess SLC8A1-AS1 expression in PTC tissues and cell lines.
  • Cell counting kit (CCK)-8 assays to evaluate cell proliferation.
  • Transwell assays to measure cell invasion and migration.
  • Western blotting and RNA immunoprecipitation assays to explore molecular interactions.

Main Results:

  • SLC8A1-AS1 expression was significantly downregulated in PTC samples and cell lines.
  • Overexpression of SLC8A1-AS1 suppressed PTC cell proliferation, invasion, and migration.
  • SLC8A1-AS1 interacted with FUS RNA Binding Protein (FUS) to maintain NUMB like endocytic adaptor protein (Numbl) mRNA stability.
  • Depletion of Numbl reversed the tumor-suppressive effects of SLC8A1-AS1.

Conclusions:

  • SLC8A1-AS1 functions as a tumor suppressor in PTC by inhibiting cell proliferation, invasion, and migration.
  • The SLC8A1-AS1/FUS/Numbl axis plays a critical role in PTC progression.
  • SLC8A1-AS1 and FUS represent potential therapeutic targets for PTC treatment.
Keywords:
FUSNumblPapillary thyroid cancerlncRNA SLC8A1-AS1progression

More Related Videos

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

8.3K
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.5K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.2K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.9K