Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protective effect and mechanisms of Buyang Huanwu decoction against hypobaric hypoxia-induced brain injury in mice: involvement of inflammatory responses and HIF-1/PI3K-Akt-related pathways.

Frontiers in immunology·2026
Same author

Visualization of Cysteine-Mediated Redox Imbalance under Environmental Exposures via π-Bridge-Tailored Fluorescent Probes.

Analytical chemistry·2026
Same author

An activatable naphthalimide-based fluorescent probe for hydrogen peroxide detection: visualizing microcystin-LR induced oxidative stress in zebrafish and hepatocyte injury mechanism.

The Analyst·2026
Same author

A donor-π-acceptor fluorescent probe for hydrogen peroxide monitoring reveals oxidative imbalance and mitochondrial damage caused by Cd/Hg exposure.

The Analyst·2026
Same author

Personalized Pathogenic Nanoplastic Coronas Orchestrate Efferocytosis-Driven Immune Evasion in Lung Adenocarcinoma.

ACS nano·2026
Same author

An integrated rotational paper-based ELISA platform for multiplex inflammatory biomarker analysis in whole blood with a DIY centrifuge.

The Analyst·2026

Related Experiment Video

Updated: Sep 22, 2025

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.7K

A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked

Dong Lin1,2,3, Bowei Li1, Longwen Fu1

  • 1CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation; Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003 Yantai, China.

Microsystems & Nanoengineering
|May 23, 2022
PubMed
Summary

Researchers developed a low-cost screen-printing method using polyurethane acrylate (PUA) to create hydrophobic barriers on nitrocellulose membranes for microfluidic paper-based analytical devices (μPADs). This enables robust point-of-care immunoassays, detecting cancer biomarkers AFP and CEA.

Keywords:
ChemistryMaterials science

More Related Videos

Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper
03:58

Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper

Published on: October 6, 2023

1.9K
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

15.9K

Related Experiment Videos

Last Updated: Sep 22, 2025

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.7K
Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper
03:58

Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper

Published on: October 6, 2023

1.9K
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

15.9K

Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Nitrocellulose (NC) membranes are vital for point-of-care immunoassays due to their protein-binding capacity.
  • Fabricating robust hydrophobic structures in NC membranes for microfluidic paper-based analytical devices (μPADs) is challenging, often requiring expensive wax printers.
  • NC membranes are prone to adhesion during printing due to electrostatic forces.

Purpose of the Study:

  • To develop a facile, fast, and low-cost strategy for fabricating μPADs in NC membranes.
  • To create durable hydrophobic barriers resistant to assay-relevant solutions.
  • To validate the PUA-based NC membrane for multiplexed immunoassays in point-of-care testing (POCT).

Main Methods:

  • Screen-printing of UV-curable polyurethane acrylate (PUA) as a barrier material on NC membranes.
  • Fabrication of microfluidic channels and reaction zones using PUA barriers.
  • Assembly of a rotational paper-based analytical device for multiplexed enzyme-linked immunosorbent assay (ELISA).

Main Results:

  • PUA barriers demonstrated resistance to surfactants and organic solvents commonly used in immunoassays.
  • The developed device successfully implemented multiplexed ELISA for alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA).
  • Achieved limits of detection for AFP (136 pg/mL) and CEA (174 pg/mL) below clinical diagnostic thresholds.

Conclusions:

  • The PUA screen-printing method offers a reliable and cost-effective approach for fabricating μPADs on NC membranes.
  • This technology provides a promising platform for point-of-care diagnostics, particularly in resource-limited settings.
  • The developed device facilitates sensitive detection of cancer biomarkers via ELISA and other bioassays.