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Updated: Sep 22, 2025
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
Published on: November 1, 2007
A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced
Shreya A Desikan1, Sailee Chavan1, Peiqi Ou1,2
1Program in Molecular and Cellular Biology, The School of Graduate Studies, State University of New York (SUNY) Downstate Health Sciences University, Brooklyn, NY 11203, USA.
Abstract:
Highly enriched germinal center (GC) B cell populations are essential for studying humoral immunity. Current MACS protocols that isolate untouched GC B cells require GC induction and typically require further FACS purification with direct antibody labeling to achieve sufficiently high purities. We present a MACS protocol with progressive and repeated negative selections that yields highly purified untouched GC B cells from both unimmunized and GC-induced mice and allows further FACS isolation of unlabeled GC B cells from remaining debris by scatter.
