Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.6K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.6K
Notch Signaling Pathway03:14

Notch Signaling Pathway

4.5K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.5K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

6.8K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-antigen-targeting T cells in pediatric central nervous system tumors: a phase 1 trial.

Nature medicine·2026
Same author

Chronic Viral Mimicry Induction following p53 Loss Promotes Immune Evasion.

Cancer discovery·2025
Same author

Diverse calcium signaling profiles regulate migratory behavior in avascular wound healing and aberrant signal hierarchy occurs early in diabetes.

American journal of physiology. Cell physiology·2024
Same author

Aberrations in Cell Signaling Quantified in Diabetic Murine Globes after Injury.

Cells·2024
Same author

Glycosaminoglycans: Roles in wound healing, formation of corneal constructs and synthetic corneas.

The ocular surface·2023
Same author

Can I count on you? Social support, depression and suicide risk.

Clinical psychology & psychotherapy·2023

Related Experiment Video

Updated: Sep 22, 2025

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
05:41

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Published on: October 6, 2022

2.7K

Age Dependent Changes in Corneal Epithelial Cell Signaling.

Kristen L Segars1, Nicholas A Azzari2, Stephanie Gomez2

  • 1Department of Pharmacology, School of Medicine, Boston University, Boston, MA, United States.

Frontiers in Cell and Developmental Biology
|May 23, 2022
PubMed
Summary

Corneal stiffness increases with age, impairing wound healing and calcium signaling. This study reveals age-related changes in corneal tissue stiffness and cell communication that reduce the efficacy of corneal repair in older mice.

Keywords:
calcium mobilizationcell-cell communicationcornealive cell imagingstiffness

More Related Videos

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures
04:48

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Published on: November 22, 2024

446
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
11:13

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

9.3K

Related Experiment Videos

Last Updated: Sep 22, 2025

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
05:41

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Published on: October 6, 2022

2.7K
Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures
04:48

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Published on: November 22, 2024

446
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
11:13

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

9.3K

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Cell Biology

Background:

  • The cornea faces daily mechanical stress, altering its extracellular matrix, stiffness, and cell junctions over time.
  • Age-related changes in corneal tissue properties may impact cellular responses to injury, particularly calcium signaling, cell motility, and wound healing.
  • Understanding these age-dependent alterations is crucial for addressing impaired corneal repair in older individuals.

Purpose of the Study:

  • To investigate the hypothesis that age-related changes in corneal stiffness affect calcium signaling post-injury and influence wound healing.
  • To compare corneal stiffness, wound healing timelines, and cellular signaling between young and aged mice.
  • To assess the role of specific purinergic receptors (P2Y2, P2X7) and pannexin-1 in cellular motility during corneal wound repair.

Main Methods:

  • Nanoindentation was used to measure the stiffness of corneal epithelium and stroma in 9-week-old (young) and 27-week-old (aged) mice.
  • Corneal wound healing was assessed by monitoring closure rates over time in both age groups.
  • Calcium signaling at wound sites was measured, and cell culture experiments with specific inhibitors (P2Y2, P2X7, pannexin-1) were conducted to evaluate their effects on cell migration and wound closure.

Main Results:

  • Significant differences in corneal stiffness were observed between young and aged mice.
  • Corneal wounds in young mice healed completely within 24 hours, while aged mice showed incomplete healing.
  • Aged mice exhibited no detectable calcium signaling at wound sites, unlike the elevated signaling in young mice. Inhibition of P2Y2, P2X7, or pannexin-1 reduced wound closure but affected cell migration differently.

Conclusions:

  • Age-related increases in corneal stiffness are associated with impaired wound healing and altered calcium signaling.
  • The findings highlight significant differences in corneal stiffness and signaling pathways that contribute to reduced wound healing efficacy in older mice.
  • Specific signaling pathways involving P2Y2, P2X7, and pannexin-1 play critical roles in corneal cell motility and repair, with age-dependent functional variations.