Related Experiment Video
Updated: Sep 22, 2025
13:28
Methodology for Sputum Induction and Laboratory Processing
Published on: December 17, 2017
27.9K
TFR1 expression in induced sputum is associated with asthma severity
Yang Wang1,2, Li Feng Gu3, Xincheng Zhao4
1Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Peerj
|May 23, 2022
Summary
Transferrin receptor 1 (TFR1) expression correlates with asthma severity and inflammatory markers in airways. Elevated TFR1 indicates more severe asthma, particularly linked to proinflammatory M1 macrophages.
Area of Science:
- Pulmonary Medicine
- Immunology
- Cell Biology
Background:
- Asthma is a chronic airway inflammatory disease.
- Iron accumulation is implicated in asthma development.
- Transferrin receptor 1 (TFR1) expression links to intracellular iron overload in macrophages.
Purpose of the Study:
- To investigate the association between TFR1 expression, macrophage phenotype, and asthma severity.
- To determine if TFR1 can serve as a biomarker for asthma severity.
Main Methods:
- Collected induced sputum from 50 asthma patients.
- Quantified mRNA expression using real-time PCR.
- Assessed inflammatory macrophage phenotype via flow cytometry.
Main Results:
- TFR1 levels inversely correlated with lung function metrics (FEV1/FVC, FEV1/VC).
- TFR1 expression positively correlated with inflammatory cytokines (IL-1β, TNF-α, IL-6, IFN-γ, IL-17A) and M1 macrophages.
- Increased TFR1 expression was associated with higher neutrophil counts and severity.
Conclusions:
- TFR1 expression is linked to asthma severity and the M1 macrophage phenotype.
- TFR1 may serve as a valuable biomarker for indicating asthma severity.
Related Concept Videos
01:26Asthma-II: Pathophysiology and Classification
2.9K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
2.9K
01:29Asthma-I: Introduction
2.8K
Asthma is a chronic respiratory ailment that requires careful management due to its varying symptoms and influencing factors. It is characterized by airway inflammation, bronchial hyperresponsiveness, and reversible airflow obstruction, leading to symptoms like wheezing, shortness of breath, chest tightness, and coughing. The symptom frequency and intensity may vary considerably over time. It is also linked to immune system responses to allergens and irritants, highlighting the complex...
2.8K
01:24Asthma-III: Symptoms and Complications
2.7K
Asthma, a common chronic respiratory condition, is classified considering the frequency and severity of symptoms alongside lung function impairment. Understanding this classification is essential for appropriate treatment and management. Here's a detailed look at the classification of asthma and its clinical features and complications:
Classification of Asthma
Classification of Asthma
2.7K
01:30Asthma-IV: Diagnostic and Management
2.7K
The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
2.7K
01:20Asthma: Pathogenesis and Management
656
Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
656
01:19Antiasthma Drugs: Leukotriene Modifiers
544
Leukotriene modifiers, or cysteinyl leukotriene receptor antagonists, are medications used to manage chronic asthma. These agents target specific inflammatory mediators produced during arachidonic acid metabolism, an essential process in generating inflammation in the body.
Leukotriene modifiers work through two distinct mechanisms:
Leukotriene modifiers work through two distinct mechanisms:
544