[Dynamic changes and effects of IL-10 secretion B cells during the progression of HIV-1/SIV disease]

Objective To investigate the dynamic changes of IL-10 secretion B cells (B10 cells) in SIVmac239-infected Rhesus macaques and the effects of B10 cells in acquired immunodeficiency syndrome progression. Methods Flow cytometry was applied to quantify CD4⁺ and CD8⁺ T lymphocytes, B cells number and the ratio of B10 cells, HLA-DR and ki67 in SIVmac39-infected Rhesus macaques. Real-time quantitative PCR was performed to detect SIV RNA levels and mRNA levels of IL-10, tumor necrosis factor-α(TNF-α) and IL-6. Dynamic changes of B10 cells in SIVmac239-infected Rhesus macaques and correlation analysis was performed with SPSS 20.0. Results SIV led to the reduction of B cells number, and comparatively increased activation and proliferation of B cells. Besides, it also caused an increase of B10 cells ratio in Rhesus macaques. No significant correlation was found between B10 cells ratio and other indicators (including CD4⁺ T cells number, TNF-α mRNA levels, ki67⁺CD4⁺T cells ratio, CTLA4⁺CD4⁺T cells ratio and CD4⁺ T cells function) in SIV-infected acute phase. However, B10 cells ratio and other indicators were in significantly negative correlation, while B10 cells ratio and SIV RNA levels were in significantly positive correlation in chronic phase. Meanwhile, no significant correlation was found between B10 cells ratio and CD8⁺ T relative indicator. Conclusion In chronic phase of SIV-infection, when B10 cells inhibits inflammation response and increases CD4⁺ T cells lose, virus replication gets uncontrolled and consequently leads to accelerated disease progression.