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Related Experiment Video

Updated: Sep 22, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

ScisorWiz: visualizing differential isoform expression in single-cell long-read data.

Alexander N Stein^1,2, Anoushka Joglekar^1,2, Chi-Lam Poon^1,2

¹Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10065, USA.

Bioinformatics (Oxford, England)

|May 23, 2022

Summary

This summary is machine-generated.

ScisorWiz visualizes RNA isoform expression across cell types and brain regions. This tool aids in understanding alternative splicing, its impact on protein function, and potential disease links.

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Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

RNA isoforms contribute to protein diversity and cellular function.
Understanding differential isoform expression across cell types is crucial for disease research.
Current methods for visualizing isoform expression are limited in scope and accessibility.

Purpose of the Study:

To introduce ScisorWiz, a novel tool for visualizing gene and RNA isoform expression.
To enable clear visualization of differential isoform expression across various cell types and tissues.
To facilitate the interpretation of single-cell long-read RNA sequencing data.

Main Methods:

ScisorWiz allows users to visualize specific genes across multiple cell types.
The tool offers various sorting and clustering options for data analysis.
It highlights features like alternative exons and single-nucleotide variants.

Main Results:

ScisorWiz provides informative and easily communicable visualizations of isoform expression.
The tool effectively depicts differential isoform expression patterns.
It aids in identifying the impact of alternative splicing on protein functionality.

Conclusions:

ScisorWiz is a key tool for interpreting single-cell long-read RNA sequencing data.
The tool is applicable to any cell type, tissue, or species.
ScisorWiz enhances understanding of isoform diversity and its role in cellular function and disease.