Related Experiment Video
Updated: Sep 22, 2025
10:52
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
Published on: March 22, 2024
1.8K
LDHA promotes osteoblast differentiation through histone lactylation
Feige Nian1, Yezhou Qian2, Fangyan Xu3
1Department of Rheumatology and Immunology, The Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, Jiaxing, Zhejiang, 314033, China.
Biochemical and Biophysical Research Communications
|May 23, 2022
Summary
Lactate dehydrogenase A (LDHA) is crucial for osteogenic differentiation by promoting histone lactylation on the JunB promoter, revealing lactate's non-canonical role in bone formation.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Osteoblast cells utilize aerobic glycolysis, converting glucose to lactate during osteogenic differentiation.
- The precise function of lactate in this process remains unclear.
- Histone lactylation, a post-translational modification, influences gene regulation and biological processes.
Purpose of the Study:
- To investigate the role of lactate dehydrogenase A (LDHA) and lactate in osteogenic differentiation.
- To elucidate the mechanism by which LDHA affects gene expression during bone formation.
Main Methods:
- Measuring lactate dehydrogenase A (LDHA), intracellular lactate, and histone lactylation levels during osteogenic differentiation.
- Utilizing LDHA knockdown and RNA-sequencing to assess effects on osteogenic differentiation and gene expression.
- Analyzing histone lactylation enrichment on the JunB promoter.
Main Results:
- LDHA expression, intracellular lactate, and histone lactylation increased during osteogenic differentiation.
- LDHA knockdown inhibited mineralized nodule formation and alkaline phosphatase (ALP) activity.
- LDHA knockdown led to decreased JunB expression, linked to reduced histone lactylation on its promoter.
Conclusions:
- LDHA plays a critical role in osteogenic differentiation through lactate production and histone lactylation.
- Histone lactylation on the JunB promoter is a key mechanism mediating LDHA's function.
- This study uncovers a novel, non-canonical role for lactate in osteogenic differentiation.
More Related Videos
06:47Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
9.5K
11:52Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
Published on: January 27, 2023
3.5K
Related Concept Videos
01:17Hormones and Bone Tissue
3.0K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.0K
01:31Osteoclasts in Bone Remodeling
3.2K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.2K
01:40Bone Remodeling
38.6K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.6K
02:32Histone Modification
14.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.2K
01:32Chromatin Modification in iPS Cells
1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
02:23Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K